Date Published:

Abstract:

This unit describes methods for isolating mouse monocytes and neutrophils, as well as in vitro protocols for measuring cell migration and polarization. The method employed here for the isolation of naive phagocytes overcomes many of the difficulties previously encountered concerning phagocyte activation. Three in vitro protocols are provided for the analysis of cell migration, one requiring no specialized equipment, one requiring the modified Boyden chamber, and the other employing a flow chamber, which measures cell adhesion, rolling, and migration. Finally, a method is provided for imaging polarized cells by confocal microscopy.

Notes:

Yona, SimonHayhoe, RichardAvraham-Davidi, InbalengResearch Support, Non-U.S. Gov'tCurr Protoc Immunol. 2010 Feb;Chapter 14:Unit 14.15. doi: 10.1002/0471142735.im1415s88.